Tissue distribution of neuropeptides and their mimetics and metabolites by means of mass spectrometry imaging technique.


Project proposed under the KNOW PhD studies in MMRC

Tissue distribution of neuropeptides and their mimetics and metabolites by means of mass spectrometry imaging technique.”

Supervisor: Andrzej W. Lipkowski, PhD, Prof.

Department of Neuropeptides

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MALDI MS imaging (MSI) is an analytical tool capable of providing spatial distribution and relative abundance of biomolecules directly in tissue. This is an excellent method for visualization of distribution in different tissues not only of endogenous but also of egzogenic compounds, and so it is especially well suited for studying the tissue distribution of endogenous peptides, and theirs synthetic mimetics and metabolites. The application of this technique is rapidly growing in the pharmaceutical science.

The goal of proposed PhD studies will be to elaborate a good and reproducible procedure for studying tissue distribution by means of MALDI-MSI technique and compare this method with the other visualization methods (like fluorescent) for studying tissue distribution of selected endogenous neuropeptides and their synthetic analogues and mimetics.

The PhD program will cover:

  • synthesis of neuropeptide peptides and their mimetics (with and without fluorescent probe)

  • enzyme degradation of studied compounds in blood plasma (HPLC-MS)

  • tissue distribution of studied compounds by means of Mass Spectrometry Imaging (MSI) what involves the following steps: (i) Tissue sectioning and preparation, (ii) Matrix deposition, (iii) Data acquisition, (iv) Data processing/image generation

  • tissue distribution of studied fluorescent compounds by means of fluorescent method

  • comparison of these two methods in studying tissues distribution of neuropeptides.

 

The candidate should first contact the Supervisor to complete the application procedure.