Department of Renal and Body Fluid Physiology

Head
Leszek Dobrowolski, Assoc. Prof., DSc - phone: +48 22 60 86 621; This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.
room B 725

Research staff
• Bożena Bądzyńska, Assoc. Prof., DSc - phone: +48 22 60 86 443, This email address is being protected from spambots. You need JavaScript enabled to view it.
• Marta Kuczeriszka, Research Assistant; DSc - phone: +48 22 60 86 443 This email address is being protected from spambots. You need JavaScript enabled to view it.
• Agnieszka Walkowska, Assoc. Prof., DSc - phone: +48 22 60 86 599; This email address is being protected from spambots. You need JavaScript enabled to view it.

PhD students
• Joanna Sitek, MSc - phone: +48 22 60 86 599; This email address is being protected from spambots. You need JavaScript enabled to view it.

Research profile
• Effectiveness of new lipofection mixtures containing polyisoprenoid derivatives in overexpressing vascular endothelial growth factor (VEGF-A): impact on blood pressure of spontaneously hypertensive rats. (Olga Gawryś)
• The mechanisms of hypotensive effectiveness of a stable analogue of 14,15-epoksyeicosatrienoic acid: EET-A; grant of National Science Center, Poland HARMONIA, 2018-2021; proj. no 2017/26/M/NZ5/00367. (Elżbieta Kompanowska-Jezierska)
• Evaluation of ang1-7 antioxidant effects in diabetes mellitus. (Marta Kuczeriszka)
• The role of interaction between P1 purine receptors and the bioavailability of nitric oxide in the regulation of intrarenal blood circulation and renal excretion in diabetes, in an animal model; grant of National Science Center, Poland OPUS, 2018-2021; proj. no 2017/25/B/NZ5/01292. (Leszek Dobrowolski)
• The renal medulla in control of renal excretion and arterial blood pressure: attemps to lower the pressure in hypertensive rats by prolonged or chronic experimental elevation of medullary blood flow. (Bożena Bądzyńska)
• Role of renal nerves in the control of intrarenal circulation and arterial blood pressure in rats with different salt intake. (Agnieszka Walkowska)

Grants

present

• The effect of chronic administration of purine P1-A2B receptor agonist, using electrospun microfibers, on intrarenal blood circulation and renal excretion during development in diabetes, in animal model. Grant of National Science Center, Poland PRELUDIUM; proj. no 2022/45/N/NZ5/02650, leader Joanna Sitek; 2023 - 2025.
• Interaction of intrarenal dopamine and endothelin-1 in the regulation of renal circulation and excretion in hypertensive rats. Grant of MMRI PAS Research Fund, proj. no. FBW 04/2022, leader Bożena Bądzyńska, 2022 - 2023.

completed

• The mechanisms of hypotensive effectiveness of a stable analogue of 14,15-epoksyeicosatrienoic acid: EET-A; grant of National Science Center, Poland HARMONIA, 2018-2021; proj. no 2017/26/M/NZ5/00367. Elżbieta Kompanowska-Jezierska; 2018-2022.
• The role of interaction between P1 purine receptors and the bioavailability of nitric oxide in the regulation of intrarenal blood circulation and renal excretion in diabetes, in an animal model; grant of National Science Center, Poland OPUS; proj. no 2017/25/B/NZ5/01292. Leszek Dobrowolski; 2018-2022.

Collaboration
foreign
•  Prof. Ludek Cervenka, Center for Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
• Prof. Louis G. Navar, Department of Physiology, Hypertension and Renal Center, Tulane University Health Sciences Center, New Orleans, Louisiana, USA.

Research equipment

  • Blood flow-meter (Transonic T106, Ithaca, NY, USA) with acoustic ultrasonic "transit-time" non-cannulating probes for determination of blood flow in arteries ≥1 mm;
  • Laser-Doppler flow-meter (Perimed 5001, Jarfala, Sweden), with surface or needle probes for determination of local tissue blood perfusion;
  • TBR4100 Free Radical Analyzer (World Precision Instruments, Sarasota, Fl, USA) for measurement of NO, H2O2, CO, H2S signal using thin gas selective electrodes placed in tissue;
  • Osmometer of Gonotec (Berlin, Germany) for measurement of osmolality in biological fluids or solutions;
  • Flame photometer of BWB Technologies Ltd (UK) for simultaneous measurement of sodium, potassium, calcium, lithium and barium concentration in biological fluids or solutions;
  • Plate reader FLUOstar Omega (BMG Labtech GmbH, Germany) - multimode microplate reader with detection mode of absorbance, fluorescence, luminescence;
  • Non-invasive blood pressure meter MRBP of IITC Life Science (Woodland Hills, CA, USA) – for the measurement of blood pressure in conscious animals (rats) by tail cuff plethysmography;
  • Non-invasive blood pressure meter CODA of Kent Scientific (Torrington, CT, USA) - for the measurement of blood pressure in conscious animals (rats or mice) by determining the tail blood volume with a sensor and an occlusion tail cuff;
  • Invasive blood pressure meter of Stoelting (USA) - for the measurement of blood pressure and heart beats in anaesthetised small animals using cannula, placed in the vessel, connected with pressure transducer.

Research methods

  • The research is focused on the interplay of vasoactive hormones and paracrine active agents (prostaglandins, CYP-450 dependent eicosanoids, NO, ATP, adenosine, bradykinin) and neural effects in the control of blood circulation in the renal cortex and medulla.
  • In our studies, we often perform the experiments with in situ kidney on anaesthetised rats in which: total renal blood flow is measured using a non-canulating ultrasonic probe placed on the renal artery, whereas for determination of the local blood perfusion in cortex, outer and inner medulla, the laser-Doppler methodology is used. In some studies tissue nitric oxide (NO) content is determined in the renal cortex and medulla using NO selective electrodes.
  • Active agents and drugs are applied by intravascular route (i.v. or into the renal artery) or into the renal tissue (interstitium) of the medulla, or cortex. To study a neural influence on renal microcirculation, an own, original method of non-invasive renal denervation is performed which enables to show immediate or early effects.
  • There are a number of studies conducted with conscious rats in which active substances are applied by intraperitoneal or subcutaneous route, also using osmotic pumps (Alzet). Other active agents are added to the diet.
  • To investigate the renal mechanism contribute to hypertension the experimental models are used. The salt content in daily diet may varied and this is of particular importance in the studies on hypertension associated with high sodium intake (sodium dependent hypertension). The rats rendered hypertensive after chronic angiontensin II infusion with osmotic pumps are used as model of hypertension associated with elevated activity of renin-angiotensin-aldosterone system. The SHR rats which developed hypertension spontaneously with age (on genetic background) are also used in our studies. It is attempted to prevent or alleviate this sort of hypertension by modification of certain metabolic processes, e.g. the synthesis of NO or of cytochrome P450-dependent pathway of arachidonic acid metabolism. Another line of research is that dealing with application of new drug carriers, ones containing polyisoprenoid alcohols, to augment the therapeutic efficacy of various drugs. Currently we start the studies with the diabetic rats (streptozotocin induced diabetes).

Selected publications

  • Kala P, …, Kompanowska-Jezierska E, Sadowski J, Walkowska A, …, Červenka L. Endothelin type A receptor blockade increases renoprotection in congestive heart failure combined with chronic kidney disease: Studies in 5/6 nephrectomized rats with aorto-caval fistula. Biomed Pharmacother. 2023; 158: 114157. doi: 10.1016/j.biopha.2022.114157.
  • Kala P, Gawrys O, …, Sadowski J, Kompanowska-Jezierska E, Walkowska A, …, Červenka L. Endothelin type A receptor blockade attenuates aorto-caval fistula-induced heart failure in rats with angiotensin II-dependent hypertension. J Hypertens. 2023; 41(1): 99-114. doi: 10.1097/HJH.0000000000003307
  • Kala P, Gawrys O, …, Sadowski J, Jezierska E Kompanowska, Walkowska A, …, Cervenka L. PS-B12-2: endothelin type a receptor blockade attenuates heart failure in rats with angiotensin ii-dependent hypertension. J Hypertens. 2023; 41(Suppl 1): p e453-e454. doi: 10.1097/01.hjh.0000917496.33970.53
  • Walkowska A, Gawrys O, Cervenka L, Kompanowska-Jezierska E. Effects of renal nerves and plasma epoxyeicosatrienoic acids on blood pressure, renal hemodynamics and excretion in spontaneously hypertensive rats. J Physiol Pharmacol. 2022; 73(2): 287-295. doi: 10.26402/jpp.2022.2.12.
  • Klopotowska M, …, Baranowska I, …, Winiarska M. PRDX-1 Supports the Survival and Antitumor Activity of Primary and CAR-Modified NK Cells under Oxidative Stress. Cancer Immunol Res. 2022; 10(2): 228-244. doi: 10.1158/2326-6066.CIR-20-1023.

  • Kuczeriszka M, Wąsowicz K. Animal models of hypertension: The status of nitric oxide and oxidative stress and the role of the renal medulla. Nitric Oxide 2022; 125-126: 40-46. doi: 10.1016/j.niox.2022.06.003.

  • Kuczeriszka M, Dobrowolski L, Walkowska A, Baranowska I, Sitek JD, Kompanowska-Jezierska E. Role of Ang1-7 in renal haemodynamics and excretion in streptozotocin diabetic rats. Clin Exp Pharmacol Physiol. 2022; 49: 432-441. doi: 10.1111/1440-1681.13618
  • Baranowska I, Gawrys O, Walkowska A, Olszynski KH, Červenka L, Falck JR, Adebesin AM, Imig JD, Kompanowska-Jezierska E. Epoxyeicosatrienoic Acid Analog and 20-HETE Antagonist Combination Prevent Hypertension Development in Spontaneously Hypertensive Rats. Front. Pharmacol. 2022; 12: 798642. doi: 10.3389/fphar.2021.798642

  • Husková Z, Kikerlová S, Sadowski J., Alánová P, Sedláková L, Papousek F, Neckár J. Increased Endogenous Activity of the Renin-Angiotensin System Reduces Infarct Size in the Rats With Early Angiotensin II-dependent Hypertension Which Survive the Acute Ischemia/Reperfusion Injury. Front Pharmacology. 2021; 12: article 679060. doi: 10.3389/fphar.2021.679060

  • Bądzyńska B., Sadowski J. Reinvestigation of the tonic natriuretic action of intrarenal dopamine: comparison of two variants of salt-dependent hypertension and spontaneously hypertensive rats. Clin  Exper Pharmacol Physiol. 2021; 49:1290-1297. doi:10.1111/1440-1681.13529

  • Bądzyńska B., Vaneckova I, Sadowski J., Hojna S, Kompanowska-Jezierska E. Effects of systemie and renal intramedullary endothelin-1 receptor blockade on tissue NO and intrarenal haemodynamics in normotensive and hypertensive rats. Eur J Pharmacol. 2021; 910, 174445. doi: 10.1016/j.eiphar.2021.174445

  • Baranowska I, Gawrys O, Roszkowska-Chojecka MM, Badzynska B, Tymecka D, Olszynski KH, Kompanowska-Jezierska E. Chymase dependent pathway of angiotensin II generation and rapeseed derived peptides for antihypertensive treatment of Spontaneously Hypertensive Rats. Front Pharmacol. 2021;12:658805. doi: 10.3389/fphar.2021.658805. 

  • Kala P, Miklovič M , Jíchová Š, Škaroupková P, Vaňourková Z, Maxová H, Gawrys O, Kompanowska-Jezierska E, Sadowski J, Imig JD, Falck JR, Veselka J, Červenka  L, Aiglová R,Vícha  M, Gloger V, Táborský M. Effects of Epoxyeicosatrienoic Acid-Enhancing Therapy on the Course of Congestive Heart Failure in Angiotensin II-Dependent Rat Hypertension: From mRNA Analysis towards Functional In Vivo Evaluation. Biomedicines. 2021;9(8):1053. doi: 10.3390/biomedicines9081053.

  • Jíchová Š, Gawryś O, Kompanowska-Jezierska E, Sadowski J, Melenovský V, Hošková  L, Červenka L, Kala P, Veselka J, Čertíková Chábová V. Kidney Response to Chemotherapy-Induced Heart Failure: mRNA Analysis in Normotensive and Ren-2 Transgenic Hypertensive Rats. Int J Mol Sci. 2021;22(16):8475. doi: 10.3390/ijms22168475

  • Bądzyńska B, Baranowska I, Sadowski J. Further evidence against the role renal medullary perfusion in short-term control of arterial pressure in normotensive and mildly or overtly hypertensive rats. Pflügers Arch - Eur J Physiol. 2021;473(4):623-631. doi: 10.1007/s00424-021-02534-1.
  • Honetschlagerová Z, Škaroupková P, Kikerlová S, Husková Z, Maxová H, Melenovský V, Kompanowska-Jezierska E, Sadowski J, Gawrys O, Kujal P, Červenka L, Čertíková Chábová V. Effects of renal sympathetic denervation on the course of congestive heart failure combined with chronic kidney disease: Insight from studies with fawn-hooded hypertensive rats with volume overload induced using aorto-caval fistula. Clin Exp Hypertens. 2021; 30:1-14. doi: 10.1080/10641963.2021.1907398
  • Roszkowska-Chojecka MM, Baranowska I, Gawrys O, Sadowski J, Walkowska A, Kalisz M, Litwiniuk A, Kompanowska-Jezierska E. Role of chymase in blood pressure control, plasma and tissue angiotensin II, renal haemodynamics, and excretion in spontaneously hypertensive rats. Clin Exp Hypertens. 2021; 9:1-10. doi: 10.1080/10641963.2021.1890762.
  • Honetschlagerová Z, Gawrys O, Jíchová Š, Škaroupková P, Kikerlová S, Vaňourková Z, Husková Z, Melenovský V, Kompanowska-Jezierska E, Sadowski J, Kolář F, Novotný J, Hejnová L, Kujal P, Červenka L. Renal sympathetic denervation attenuates congestive heart failure in angiotensin II-dependent hypertension: Studies with Ren-2 transgenic hypertensive rats with aortocaval fistula. Kidney Blood Press Res. 2021;46(1):95-113. doi: 10.1159/000513071. Epub 2021 Feb 2.
  • Walkowska A, Červenka L, Imig JD, Falck JR, Sadowski J, Kompanowska-Jezierska E. Early Renal Vasodilator and Hypotensive Action of Epoxyeicosatrienoic Acid Analog (EET-A) and 20-HETE Receptor Blocker (AAA) in Spontaneously Hypertensive Rats. Front Physiol. 2021;12:622882. doi: 10.3389/fphys.2021.622882.
  • Gawrys O, Rak M, Baranowska I, Bobis-Wozowicz S, Szaro K, Madeja Z, Swiezewska E, Masnyk M, Chmielewski M, Karnas E, Kompanowska-Jezierska E. Polyprenol-Based Lipofecting Agents for In Vivo Delivery of Therapeutic DNA to Treat Hypertensive Rats. Biochem Genet. 2021;59(1):62-82. doi: 10.1007/s10528-020-09992-9.
  • Kuczeriszka M, Sitek JD, Walkowska A, Sadowski J, Dobrowolski L. Interplay of the adenosine system and NO in control of renal haemodynamics and excretion: comparison of normoglycaemic and streptozotocin diabetic rats. Nitric Oxide 2020, w druku
  • Gawrys O, Husková Z, Baranowska I, Walkowska A, Sadowski J, … Červenka L, …, Kompanowska-Jezierska E. Combined treatment with epoxyeicosatrienoic acid analog and 20-hydroxyeicosatetraenoic acid antagonist provides substantial hypotensive
    effect in spontaneously hypertensive rats. J Hypertens. 2020;38(9):1802-1810. doi: 10.1097/HJH.0000000000002462. PMID: 32384390.
  • Gawrys O, Rak M, Baranowska I, …, Swiezewska E, ….., Kompanowska-Jezierska E. Polyprenol-Based Lipofecting Agents for In Vivo Delivery of Therapeutic DNA toTreat Hypertensive Rats. Biochem Genet. 2020. https://doi.org/10.1007/s10528-020-09992-9. PMID: 32767051.
  • Kosson A, Kosson P, Kuczeriszka M. Procedury awaryjne w celu zapewnienia dobrostanu zwierząt laboratoryjnych. Przegląd Hodowlany 2020;4:29-33.
  • Gawrys-Kopczynska M, Konop M,…., Dobrowolski L, …., Ufnal M. TMAO, a seafood-derived molecule, produces diuresis and reduces mortality in heart failure rats. Elife. 2020 Jun 8; 9: e57028. doi: 10.7554/eLife.57028. PMID: 32510330.
  • Caruso F, ... , Sitek JD, Gionfra F, Pedersen JZ, Incerpi S. Antioxidant properties of embelin in cell culture. Electrochemistry and theoretical mechanism of scavenging. Potential scavenging of superoxide radical through the cell membrane. Antioxidants (Basel). 2020;9(5):382. doi: 10.3390/antiox9050382. PMID: 32380755.
  • Sadowski J, Bądzyńska B: Altered renal medullary blood flow: A key factor or a parallel event in control of sodium excretion and blood pressure? [published online ahead of print, 2020 Mar 12]. Clin Exp Pharmacol Physiol. 2020;47(8):1323-1332. https://doi.org/10.1111/1440-1681.13303
  • Bądzyńska B, Zakrocka I, Turski WA, Olszyński KH, Sadowski J, Kompanowska-Jezierska E. Kynurenic acid selectively reduces heart rate in spontaneously hypertensive rats. Naunyn Schmiedebergs Arch Pharmacol. 2020;393(4):673-679. doi: 10.1007/s00210-019-01771-7.
  • Čertíková Chábová V, Kujal P, Vaňourková Z, Škaroupková P, Sadowski J, Kompanowska-Jezierska E, Tesař V, Hammock B, Imig J, Maxová H, Červenka L, Vaněčková I. Addition of Endothelin A-Receptor Blockade Spoils the Beneficial Effect of Combined Renin-Angiotensin and Soluble Epoxide Hydrolase Inhibition: Studies on the Course of Chronic Kidney Disease in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats. Kidney Blood Press Res. 2019; 44(6): 1493-1505. doi: 10.1159/000504137. Epub 2019 Nov 26. PMID: 31770762.
  • Vaněčková I, Hojná S, Vernerová Z, Kadlecová M, Rauchová H, Kompanowska-Jezierska E, Vaňourková Z, Červenka L, Zicha J: Renoprotection Provided by Additional Diuretic Treatment in Partially Nephrectomized Ren-2 Transgenic Rats Subjected to the Combined RAS and ETA Blockade. Front Physiol. 2019 Sep 18;10:1145. doi: 10.3389/fphys.2019.01145. eCollection 2019.
  • Walkowska A, Vaněčková I, Sadowski J, Kompanowska-Jezierska E. Rapid blood pressure increase after renal denervation in anaesthetized rats: interaction of oxidative stress and neuronal nitric oxide synthase. J Physiol Pharmacol. 2019 Aug;70(4). doi: 10.26402/jpp.2019.4.12. Epub 2019 Nov 15.
  • Vacková Š, Kikerlová S, Melenovsky V, Kolář F, Imig JD, Kompanowska-Jezierska E, Sadowski J, Červenka L. Altered Renal Vascular Responsiveness to Vasoactive Agents in Rats with Angiotensin II-Dependent Hypertension and Congestive Heart Failure. Kidney Blood Press Res. 2019;44(4):792-809. doi: 10.1159/000501688.
  • Vaněčková I, Hojná S, Kadlecová M, Kompanowska-Jezierska E, Zicha J. Distinct effects of bosentan on NO-dependent vasodilation and calcium influx in heterozygous Ren-2 transgenic rats on high-salt diet. Physiol Res. 2019 Oct 25;68(5):717-725. Epub 2019 Aug 19. PMID: 31424254.
  • Kuczeriszka M, Walkowska A, Olszynski KH, Rafalowska J, Sadowski J, Kompanowska-Jezierska E: Arginine and tetrahydrobiopterin supplementation in rats with salt-induced blood pressure increase: minor hypotensive effect but improvement of renal haemodynamics. J Physiol Pharmacol. 2019; doi: 10.26402/jpp.2019.2.05. Epub 2019 Jul 22. PubMed PMID: 31356183.
  • Kala P, Červenka L, Škaroupková P, Táborský M, Kompanowska-Jezierska E, Sadowski J.
    Sex-linked differences in the mortality in Ren-2 transgenic hypertensive rats with aorto-caval fistula: effects of treatment with angiotensin converting enzyme alone and combined with inhibitor of soluble epoxide hydrolase. Physiol Res. 2019. [Epub ahead of print] PubMed PMID: 31177795.
  • Pokorný M, Mrázová I, Kubátová H, Piťha J, Malý J, Pirk J, Maxová H, Melenovský V, Šochman J, Sadowski J, Červenka L, Čermák Z, Volenec K, Netuka I. Intraventricular placement of a spring expander does not attenuate cardiac atrophy of the healthy heart induced by unloading via heterotopic heart transplantation. Physiol Res. 2019. [Epub ahead of print] PubMed PMID: 31177788.
  • Vacková Š, Kopkan L, Kikerlová S, Husková Z, Sadowski J, Kompanowska-Jezierska E, Hammock BD, Imig JD, Táborský M, Melenovský V, Červenka L. Pharmacological Blockade of Soluble Epoxide Hydrolase Attenuates the Progression of Congestive Heart Failure Combined With Chronic Kidney Disease: Insights From Studies With Fawn-Hooded Hypertensive Rats. Front Pharmacol. 2019; doi: 10.3389/fphar.2019.00018. eCollection 2019.
  • Bądzyńska B, Baranowska I, Gawryś O, Sadowski J. Evidence against a crucial role of renal medullary perfusion in blood pressure control of hypertensive rats. J Physiol. 2019;597(1): 211-223.
  • Kratky V, Kopkan L, Kikerlova S, Huskova Z, Taborsky M, Sadowski J, Kolar F,Cervenka L. The Role of Renal Vascular Reactivity in the Development of Renal Dysfunction in Compensated and Decompensated Congestive Heart Failure. Kidney Blood Press Res. 2018;43(6):1730-41.
  • Sedláková L, Kikerlová S, Husková Z, Červenková L, Chábová VČ, Zicha J, Falck JR, Imig JD, Kompanowska-Jezierska E, Sadowski J, Krátký V, Červenka L, Kopkan L. 20-Hydroxyeicosatetraenoic acid antagonist attenuates the development of malignant hypertension and reverses it once established: a study in Cyp1a1-Ren-2 transgenic rats. Biosci Rep. 2018;38(5):BSR20171496. doi: 10.1042/BSR20171496.
  • Roszkowska-Chojecka MM, Walkowska A, Sadowski J, Dobrowolski L. Clopidogrel partially counteracts adenosine-5'-diphosphate effects on blood pressure and renal hemodynamics and excretion in rats. Am J Med Sci. 2018;36(3):287-295.
  • Kala P, Sedláková L, Škaroupková P, Kopkan L, Vaňourková Z, Táborský M, Nishiyama A, Hwang SH, Hammock BD, Sadowski J, Melenovský V, Imig JD, Červenka L. Effect of angiotensin-converting enzyme blockade, alone or combined with blockade of soluble epoxide hydrolase, on the course of congestive heart failure and occurrence of renal dysfunction in Ren-2 transgenic hypertensive rats with aorto-caval fistula. Physiol Res. 2018;67(3):401-415.
  • Pokorný M, Mrázová I, Šochman J, Melenovský V, Malý J, Pirk J, Červenková L, Sadowski J, Čermák Z, Volenec K, Vacková Š, Maxová H, Červenka L, Netuka I. Isovolumic loading of the failing heart by intraventricular placement of a spring expander attenuates cardiac atrophy after heterotopic heart transplantation. Biosci Rep. 2018. pii: BSR20180371.
  • Červenka L, Husková Z, Kopkan L, Kikerlová S, Sedláková L, Vaňourková Z, Alánová P, Kolář F, Hammock BD, Hwang SH, Imig JD, Falck JR, Sadowski J, Kompanowska-Jezierska E, Neckář J. Two pharmacological epoxyeicosatrienoic acid-enhancing therapies are effectively antihypertensive and reduce the severity of ischemic arrhythmias in rats with angiotensin II-dependent hypertension. J Hypertens. 2018;36(6):1326-1341.
  • Čertíková Chábová V, Kujal P, Škaroupková P, Varňourková Z, Vacková Š, Husková Z, Kikerlová S, Sadowski J, Kompanowska-Jezierska E, Baranowska I, Hwang SH, Hammock BD, Imig JD, Tesař V, Červenka L: Combined Inhibition of Soluble Epoxide Hydrolase and Renin-Angiotensin System Exhibits Superior Renoprotection to Renin-Angiotensin System Blockade in 5/6 Nephrectomized Ren-2 Transgenic Hypertensive Rats with Established Chronic Kidney Disease. Kidney Blood Press Res. 2018;43(2):329-49.
  • Pokorný M, Mrázová I, Malý J, Pirk J, Netuka I, Vaňourková Z, DoleŽelová Š, Červenková L, Maxová H, Melenovský V, Šochman J, Sadowski J, Červenka L. Effects of increased myocardial tissue concentration of myristic, palmitic and palmitoleic acids on the course of cardiac atrophy of the failing heart unloaded by heterotopic transplantation. Physiol Res. 2018;67(1):13-30.
  • Kuczeriszka M, Kompanowska-Jezierska E, Sadowski J, Prieto MC, Navar LG. Modulating Role of Ang1-7 in Control of Blood Pressure and Renal Function in AngII-infused Hypertensive Rats. Am J Hypertens. 2018;31(4):504-11.
  • Gawrys O, Baranowska I, ..., Kompanowska-Jezierska E. Innovative lipid-based carriers containing cationic derivatives of polyisoprenoid alcohols augment antihypertensive effectiveness of candesartan in spontaneously hypertensive rats. Hypertens Res. 2018;41(4):234-45.
  • Jaworska K, Huc T, Samborowska E, Dobrowolski L, BielinskaK, Gawlak M, Ufnal M. Hypertension in rats is associated with an increased permeability of the colon to TMA, a gut bacteria metabolite. PLoS One. 2017 Dec 13;12(12): e0189310.
  • Sporková A, ..., Kompanowska-Jezierska E, Sadowski J, Maxová H, Červenka L. Fenofibrate Attenuates Hypertension in Goldblatt Hypertensive Rats: Role of 20-Hydroxyeicosatetraenoic Acid in the Nonclipped Kidney. Am J Med Sci. 2017;353(6):568-79.
  • Walkowska A, Pawlak M, Jane SM, Kompanowska-Jezierska E, Wilanowski T: Effects of high and low sodium diet on blood pressure and heart rate in mice lacking the functional grainyhead-like 1 gene. Physiol Res. 2017;66(1):163-65.
  • Sporková A, Husková Z, Škaroupková P, Rami Reddy N, Falck JR, Sadowski J, Červenka L. Vasodilatory responses of renal interlobular arteries to epoxyeicosatrienoic acids analog are not enhanced in Ren-2 transgenic hypertensive rats: evidence against a role of direct vascular effects of epoxyeicosatrienoic acids in progression of experimental heart failure. Physiol Res. 2017; 31;66(1): 29-39.
  • Sedláková L, ..., Sadowski J, Kompanowska-Jezierska E, ..., Červenka L: Renin-angiotensin system blockade alone or combined with ET(A) receptor blockade: effects on the course of chronic kidney disease in 5/6 nephrectomized Ren-2 transgenic hypertensive rats. Clin Exp Hypertens. 2017;39(2):183-195.
  • Bądzyńska B, Lipkowski A.W, Sadowski J. An antihypertensive opioid: Biphalin, a synthetic non-addictive enkephalin analog decreases blood pressure in spontaneously hypertensive rats. Pharmacol Rep. 2016; 68(1): 51-55.
  • Kuczeriszka M, Lipkowski AW, Sadowski J, Kompanowska-Jezierska E. An endomorphine analog ([d-Ala2]-Endomorphin 2, TAPP) lowers blood pressure and enhances tissue nitric oxide in anesthetized rats. Pharmacol Rep. 2016;68(3):616-619.
  • Husková Z, ....., Kompanowska-Jezierska E, Sadowski J, Kramer HJ, Červenka L: Intrarenal alterations of the angiotensin-converting enzyme type 2/angiotensin 1-7 complex of the renin-angiotensin system do not alter the course of malignant hypertension in Cyp1a1-Ren-2 transgenic rats. Clin Exp Pharmacol Physiol. 2016;43(4):438-449.
  • Sporková A, Reddy RN, Falck JR, Imig JD, Kopkan L, Sadowski J, Červenka L. Interlobular Arteries From 2-Kidney, 1-Clip Goldblatt Hypertensive Rats' Exhibit-Impaired Vasodilator Response to Epoxyeicosatrienoic Acids. Am J Med Sci. 2016;351(5):513-519.
  • Červenka L, Škaroupková P, Kompanowska-Jezierska E, Sadowski J. Sex-linked differences in the course of chronic kidney disease and congestive heart failure: a study in 5/6 nephrectomized Ren-2 transgenic hypertensive rats with volume overload induced using aorto-caval fistula. Clin Exp Pharmacol Physiol. 2016;43(10):883-895.
  • Jíchová Š, ...., Sadowski J, Kompanowska-Jezierska E, ...., Imig JD, Červenka L. Epoxyeicosatrienoic acid analog attenuates the development of malignant hypertension, but does not reverse it once established: a study in Cyp1a1-Ren-2 transgenic rats. J Hypertens. 2016;34(10):2008-2025.
  • Bądzyńska B, Lipkowski AW, Olszyński KH, Sadowski J. Different blood pressure responses to opioids in 3 rat hypertension models: role of the baseline status of sympathetic and renin-angiotensin systems. Can J Physiol Pharmacol. 2016;94(11):1159-1169.
  • Doleželová Š, Jíchová Š, ..., Sadowski J, Červenka L, Kopkan L. Progression of hypertension and kidney disease in aging fawn-hooded rats is mediated by enhanced influence of renin-angiotensin system and suppression of nitric oxide system and epoxyeicosanoids. Clin Exp Hypertens. 2016;26:1-8.
  • Kuczeriszka M, Dobrowolski L, Walkowska A, Sadowski J. Influence of P2X receptors on renal medullary circulation is not altered by angiotensin II pretreatment. Pharmacol Rep. 2016; 68: 1230-36.
  • Tomasova L, Dobrowolski L, ...., Ostaszewski R, Ufnal M. Intracolonic hydrogen sulfide lowers blood pressure in rats. Nitric Oxide 2016;22;60:50-58.
  • Jíchová Š, Doleželová Š, Kopkan L, Kompanowska-Jezierska E, Sadowski J, Červenka L. Fenofibrate Attenuates Malignant Hypertension by Suppression of the Renin-angiotensin System: A Study in Cyp1a1-Ren-2 Transgenic Rats. Am J Med Sci. 2016;352(6):618-630.
  • Dobrowolski L, Kuczeriszka M, Castillo A, Majid DS, Navar LG. Role of atrial natriuretic peptide in mediating the blood pressure-independent natriuresis elicited by systemic inhibition of nitric oxide. Pflugers Arch. 2015;467(4):833-841.
  • Hampl V, Herget J, Bíbová J, ..., Sadowski J, Červenka L. Intrapulmonary activation of the angiotensin-converting enzyme type 2/angiotensin 1-7/G-protein-coupled Mas receptor axis attenuates pulmonary hypertension in Ren-2 transgenic rats exposed to chronic hypoxia. Physiol Res. 2015;64(1):25-38.
  • Pawlak M, Walkowska A, ..., Kompanowska-Jezierska E, Wilanowski T. Consequences of the loss of the Grainy head-like 1 gene for renal gene expression, regulation of blood pressure and heart rate in a mouse model. Acta Bioch Pol. 2015;62(2):287-296.
  • Roszkowska-Chojecka MM, Walkowska A, Gawryś O, Baranowska I, ..., Kompanowska-Jezierska E. Effects of chymostatin, a chymase inhibitor, on blood pressure, plasma and tissue angiotensin II, renal haemodynamics and renal excretion in two models of hypertension in the rat. Exp Physiol. 2015;100(9):1093-1105.
  • Walkowska A, Kuczeriszka M, Sadowski J, Olszyński KH, Dobrowolski L, Červenka L, Hammock BD, Kompanowska-Jezierska E. High salt intake increases blood pressure in normal rats: putative role of 20-HETE and no evidence on changes in renal vascular reactivity. Kidney Blood Press Res. 2015;40(3):323-342.
  • Bądzyńska B, Lipkowski AW, Sadowski J, Kompanowska-Jezierska E. Vascular effects of a tripeptide fragment of novokinine in hypertensive rats: Mechanism of the hypotensive action. Pharmacol Rep. 2014;66(5):856-861.
  • Gawryś O, Polkowska M, Roszkowska-Chojecka MM, ..., Kompanowska-Jezierska E. Effects of liposomes with polyisoprenoids, potential drug carriers, on the cardiovascular and excretory system in rats. Pharmacol Rep. 2014; 66(2): 273-78.
  • Gawrys O, Olszynski KH, Gawarecka, K, Swiezewska E, Masnyk M, Kompanowska-Jezierska E. Cationic derivative of polyprenol, a potential component of liposomal drug carriers, does not alter renal function in rats. Eur J Lipid Sci Tech. 2014;116(5):659-662
  • Sporková A, ...., Kompanowska-Jezierska E, Sadowski J, Kramer HJ, Červenka L: Different mechanisms of acute versus long-term antihypertensive effects of soluble epoxide hydrolase inhibition: studies in Cyp1a1-Ren-2 transgenic rats. Clin Exp Pharmacol Physiol. 2014;41(12):1003-1013.
  • Kuczeriszka M, Dobrowolski L, Walkowska A, Sadowski J, Kompanowska-Jezierska E. Adenosine Effects on Renal Function in the Rat: Role of sodium Intake and Cytochrome P450. Nephron Physiol. 2013; 123: 1-5.
  • Walkowska A, Sadowski J, Kompanowska-Jezierska E: Oxidative stress and neuronal NOS activity: putative determinants of rapid hypertensive response to renal denervation in anaesthetised rats. Physiol Res. 2013; 32: 257-66.

Patents
2017 – patent PAT.226845 on invention no. P-406517 “Kynureic acid application in the treatment of disturbances in the body water-electrolyte homeostasis”. Application no: P-406517, date of application: 13.12.2013, obtaining: 03.04.2017 r.