Laboratory of Ischemic and Neurodegenerative Brain Research

Head
Prof. Ryszard Pluta, MD, PhD - phone. +48 / 22 60 86 540/469, This email address is being protected from spambots. You need JavaScript enabled to view it.

Technical staff
Sławomir Januszewski - phone. +48 22 60 86 469, This email address is being protected from spambots. You need JavaScript enabled to view it.

Research profile
• Complete brain ischemia: neuropathology, changes in genome and proteome
• Analysis of genes and proteins associated with Alzheimer's disease after brain ischemia
• Searching for common pathological mechanisms in ischemic brain and Alzheimer's disease
• Development of an ischemic model of Alzheimer's disease

Grants
• Method of producing a standardized rich polyphenolic nutraceutical, for use in the prevention of neurodegenerative diseases. Tango 2. The National Centre for Research and Development. prof. Anna Bogucka-Kocka (Main performer-Prof. R. Pluta) 04.2017-03.2020.
• Looking for the same pathological factors in brain ischemia and Alzheimer's disease: Genes. DEC-2013/09/B/NZ7/01345. National Science Centre. Prof. R. Pluta. 02.2014-02.2017.

Colaboration
domestic
• prof. Stanisław Jerzy Czuczwar, Medical University of Lublin, Lublin.
• prof. Anna Bogucka-Kocka, Medical University of Lublin, Lublin.
• prof. Janusz Kocki, Medical University of Lublin, Lublin.
• prof. Wanda Małgorzata Furmaga-Jabłońska, Medical University of Lublin Lublin.

foreign
• prof. Pavle Andjus, University of Belgrade, Belgrade, Serbia.

Research equipment
• Equipment for conducting experiments on rodents.
Research methods
• An experimental model of complete brain ischemia in the rat.

Selected publications

  • Radenovic L, Nenadic M, Ułamek-Kozioł M, Januszewski S, Czuczwar SJ, Andjus PR, Pluta R. Heterogeneity in brain distribution of activated microglia and astrocytes in a rat ischemic model of Alzheimer’s disease after 2 years of survival. Aging (Albany NY). 2020;12:12251-12267.
  • Pluta R, Ułamek-Kozioł M, Januszewski S, Czuczwar SJ. Participation of amyloid and tau protein in neuronal death and neurodegeneration after brain ischemia. Int J Mol Sci., 2020;21:4599.
  • Pluta R, Ułamek-Kozioł M, Kocki J, Bogucki J, Januszewski S, Bogucka-Kocka A, Czuczwar SJ. Expression of the tau protein and amyloid protein precursor processing genes in the CA3 area of the hippocampus in the ischemic model of Alzheimer's disease in the rat. Mol Neurobiol., 2020;57:1281–1290.
  • Pluta R, Ułamek-Kozioł M, Januszewski S, Czuczwar SJ. Shared genomic and proteomic contribution of amyloid and tau protein characteristic of Alzheimer's disease to brain ischemia. Int J Mol Sci., 2020;21:3186.
  • Ułamek-Kozioł M, Czuczwar SJ, Januszewski S, Pluta R. Proteomic and genomic changes in tau protein, which are associated with Alzheimer's disease after ischemia-reperfusion brain injury. Int J Mol Sci., 2020;21:892.
  • Pluta R (Ed.) Brain ischemia: Alzheimer’s disease mechanisms. Nova Science Publishers, Inc. New York. USA. 2019.
  • Ułamek-Kozioł M, Czuczwar SJ, Kocki J, Januszewski S, Bogucki J, Bogucka-Kocka A, Pluta R. Dysregulation of autophagy, mitophagy, and apoptosis genes in the CA3 region of the hippocampus in the ischemic model of Alzheimer's disease in the rat. J Alzheimers Dis., 2019;72:1279-1286.
  • Pluta R, Bogucka-Kocka A, Ułamek-Kozioł M, Bogucki J, Januszewski S, Kocki J, Czuczwar SJ. Ischemic tau protein gene induction as an additional key factor driving development of Alzheimer's phenotype changes in CA1 area of hippocampus in an ischemic model of Alzheimer's disease. Pharmacol Rep., 2018;70:881-884.
  • Ułamek-Kozioł M, Kocki J, Bogucka-Kocka A, Januszewski S, Bogucki J, Czuczwar SJ, Pluta R. Autophagy, mitophagy and apoptotic gene changes in the hippocampal CA1 area in a rat ischemic model of Alzheimer's disease. Pharmacol Rep., 2017;69:1289-1294.
  • Pluta R, Kocki J, Ułamek-Kozioł M, Petniak A, Gil-Kulik P, Januszewski S, Bogucki J, Jabłoński M, Brzozowska J, Furmaga-Jabłońska W, Bogucka-Kocka A, Czuczwar SJ. Discrepancy in expression of β-secretase and amyloid-β protein precursor in Alzheimer-related genes in the rat medial temporal lobe cortex following transient global brain ischemia. J Alzheimers Dis., 2016;51:1023-1031.
  • Ułamek-Kozioł M, Kocki J, Bogucka-Kocka A, Petniak A, Gil-Kulik P, Januszewski S, Bogucki J, Jabłoński M, Furmaga-Jabłońska W, Brzozowska J, Czuczwar SJ, Pluta R. Dysregulation of autophagy, mitophagy and apoptotic genes in the medial temporal lobe cortex in an ischemic model of Alzheimer’s disease. J Alzheimers Dis., 2016;54:113-121.
  • Pluta R, Kocki J, Ułamek-Kozioł M, Bogucka-Kocka A, Gil-Kulik P, Januszewski S, Jabłoński M, Petniak A, Brzozowska J, Bogucki J, Furmaga-Jabłońska W, Czuczwar SJ. Alzheimer-associated presenilin 2 gene is dysregulated in rat medial temporal lobe cortex after complete brain ischemia due to cardiac arrest. Pharmacol Rep., 2016;68:155-161.
  • Kocki J, Ułamek-Kozioł M, Bogucka-Kocka A, Januszewski S, Jabłoński M, Gil-Kulik P, Brzozowska J, Petniak A, Furmaga-Jabłońska W, Bogucki J, Czuczwar SJ, Pluta R. Dysregulation of amyloid precursor protein, β-secretase, presenilin 1 and 2 genes in the rat selectively vulnerable CA1 subfield of hippocampus following transient global brain ischemia. J Alzheimers Dis., 2015;47:1047–1056.
  • Pluta R, Jabłoński M, Ułamek-Kozioł M, Kocki J, Brzozowska J, Januszewski S, Furmaga-Jabłońska W, Bogucka-Kocka A, Maciejewski R, Czuczwar SJ. Sporadic Alzheimer's disease begins as episodes of brain ischemia and ischemically dysregulated Alzheimer's disease genes. Mol Neurobiol., 2013;48:500-515.
  • Pluta R, Furmaga-Jabłońska W, Maciejewski R, Ułamek-Kozioł M, Jabłoński M. Brain ischemia activates β- and γ-secretase cleavage of amyloid precursor protein: Significance in sporadic Alzheimer’s Disease. Mol Neurobiol., 2013;47:425-434.
  • Sekeljic V, Bataveljic D, Stamenkovic S, Ułamek M, Jabłoński M, Radenovic L, Pluta R, Andjus PR. Cellular markers of neuroinflammation and neurogenesis after ischemic brain injury in the long-term survival rat model. Brain Struct Funct., 2012;217:411-420.
  • Kiryk A, Pluta R, Figiel I, Mikosz M, Ułamek M, Niewiadomska G, Jabłoński M, Kaczmarek L. Transient brain ischemia due to cardiac arrest causes irreversible long-lasting cognitive injury. Behav Brain Res., 2011;219:1-7.
  •  Jabłoński M, Maciejewski R, Januszewski S, Ułamek M, Pluta R. One year follow up in ischemic brain injury and the role of Alzheimer factors. Physiol Res., 2011;60(Suppl. 1.): S113-S119.
  • Pluta R, Januszewski S, Jabłoński M, Ułamek M. Factors in creepy delayed neuronal death in hippocampus following brain ischemia-reperfusion injury with long-term survival. Acta Neurochir., 2010;106(Suppl.):37-41.
  • Pluta R, Ułamek M, Jabłoński M. Alzheimer’s mechanisms in ischemic brain degeneration. Anat Rec., 2009;292:1863-1881.
  • Pluta R (Ed.) Ischemia-reperfusion pathways in Alzheimer’s disease. Nova Science Publishers, Inc. New York. USA. 2007.